HIF-1α may accommodate the ability of forming vasculogenic mimicry in esophageal squamous cell carcinoma by regulating VE-cadherin, which affects VM formation through EphA2 and LN5γ2. Conclusion: HIF-1α up-regulation of VE-cadherin may be a critical molecular event involved in the vasculogenic mimicry and esophageal squamous cell carcinoma formation. Key Word(s): 1. HIF-1alpha; 2. VE-cadherin; 3. vasculogenic mimicry; 4. esophageal carcinoma; Presenting Author: XIANGMING FANG Corresponding Author: XIANGMING FANG Affiliations: wuhan

puren hospital Objective: To investigate the relationship between cyclin D1 gene polymorphism and susceptibility of gastric cancer and evaluated the combined effect of cyclin D1 gene polymorphism and Helicobacter pylori (Hp) infection on gastric cancer. Methods: By polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP), the cyclin D1 (A870G) genotyping was performed among 115 cases with gastric buy Selisistat cancer and 112 controls with chronic gastritis. MG-132 datasheet ELISA was adapted to examine Hp infection of the above people. Results: There was significant difference of cyclin D1 genotypes frequencies between patients with gastric cancer and controls (P < 0.05).

The subjects carrying AA genotype were at an increase risk of gastric cancer compared to subjects carrying A/G and GG genotype with an odds ratio (OR) of 2.60 (95%CI, 1.47∼4.58). In patients Hp infection, the difference of cyclin D1 genotypes frequencies between patients with gastric cancer and controls was statistically significance (P < 0.05). The risk for gastric cancer is higher in AA genotype carriers with Hp infection than in A/G and GG genotype carriers with Hp infection, with OR of 3.82(95%CI, 1.92∼7.61). Conclusion: The cyclin D1 gene polymorphism

is related to the genetic susceptibility of gastric cancer. Individuals click here with cyclin D1 AA genotype show an increased risk for gastric cancer. Association of the cyclin D1 AA genotype with Hp infection could significantly increase gastric cancer risk. Key Word(s): 1. Stomach neoplasms; 2. cyclin D1; 3. Gene polymorphism; 4. Helicobacter pylori; Presenting Author: XIAOMEI ZHANG Additional Authors: YUNSHENG YANG, CHAO YANG, GANG SUN Corresponding Author: YUNSHENG YANG Affiliations: Chinese PLA General Hospital Objective: Therapies for esophageal cancer primarily rely on surgery and radiotherapy. But the prognosis is poor in patients with advanced stage. Previous reports have suggested that treatment with cytokine-induced killer (CIK) cells may benefit patients with various types of tumor. However, CIK-based immunotherapy is rarely used in those patients. In this study, effects of CIK cells against human esophageal squamous cancer were evaluated in vitro and in vivo. Methods: CIK cells were generated routinely from human peripheral blood mononuclear cells (PBMCs) in the presence of CD3, IFN-γ and IL-2 in vitro. The phenotype of CIK cells was analyzed by fluorescence-activated cell sorting.