We have confirmed by sequence analysis that this gene

is 100% identical ISRIB order to that in the wild-type strain NRRL 1951, indicating that further industrial strain improvement steps have not modified the sequence of this gene. We have termed this gene ial because it encodes a protein (IAL for IAT-Like) that shares a 54% similarity (E-value 6e-43, 34% identity) and a 52% similarity (E-value 5e-42, 35% identity) with the IATs of P. chrysogenum and A. nidulans, respectively. In addition, the IAL showed 81% similarity with an unnamed protein product from A. oryzae (GenBank: BAE55742), 80% similarity with a putative IAT of A. clavatus (GenBank: XP_001271254), 79% similarity with the hypothetical protein An02g08570 from A. niger (GenBank: XP_001399990), 78% similarity with a predicted protein from A. terreus (GenBank: XP_001213312), 76% similarity with a putative IAT from Neosartorya fischeri (GenBank: XP_001263202), 76% similarity with a putative IAT from www.selleckchem.com/products/tpx-0005.html A. fumigatus (GenBank: XP_754359) and 60% similarity with the hypothetical protein AN6775.2 of A. nidulans

(GenBank: XP_664379), among others (Fig. 1). The IAL protein is present in several of the sequenced genomes of ascomycetes and deuteromycetes. Figure 1 Alignment of the P. EGFR inhibitor chryosogenum IAL (IALPc) to the IATs of P. chrysogenum (IATPc) and A. nidulans (IATAn) and to different homologues of the IAL present in filamentous fungi such as A. clavatus (Aclava), A. fumigatus (Afumig), A. nidulans (Anidul), A. niger (Aniger), A. oryzae (Aoryzae), RANTES A. terreus (Aterreus) and N. fischeri (Nfischeri). Those motifs or residues important for IAT enzyme processing or activity are boxed. It is noteworthy

that the P. chrysogenum IAL shows some important amino acids and domains that are present in the wild-type IAT, such as the 104 DGCTS 108 motif (equivalent to the 101 DGCTT 105 motif of the IAT containing the G102-C103 processing site) and the S231, which is equivalent to the IAT S227 residue required for IAT cleavage and activity [20]. However, the peroxisomal targeting sequence (PTS1) is absent from the C’-end of the P. chrysogenum IAL and related proteins from other filamentous fungi, unlike what is observed in the P. chrysogenum and A. nidulas IATs, which bear the PTS1 ARL and ANI motifs, respectively (Fig. 1). Penicillin biosynthesis is not affected in the ial null mutant In order to test whether the IAL protein participates in the biosynthesis of penicillin in P. chrysogenum, we studied the function of the gene in a penicillin high-producing strain, DS17690 [28]. In order to generate null mutants in the ial gene without disturbing the genomic context, the amdS marker was inserted between the ial promoter and its ORF, in the opposite orientation (see Fig. 2). To increase the rate of homologous targeting, a derivative of P.