5% bovine serum albumin in PBS mixed with 0. 1% Triton for 1 h at room temperature and incubated with the indicated primary antibodies in 2% goat serum PBS 0. 1% Triton overnight. After rinsing in PBS, sections www.selleckchem.com/products/Imatinib(STI571).html were incubated with the corresponding secondary antibodies for 1 h and washed 4 times. Slices were incubated with 300 nM DAPI in PBS for 2 min, washed and mounted with Citifluor. Images from 12 to 15 cryoslices from 3 different preparations Inhibitors,Modulators,Libraries were acquired using a Zeiss Axioplan 2 microscope and digital Axiocam camera. AxioVision software was used to standardize the images by setting all the parameters to a constant value. Western blotting Slice cultures were collected and homogenized on ice in a lysis buffer mixed with phosphatase inhibitor cocktail tab lets and complete prote ase inhibitor mix.

Protein concentration was determined using the BCA protein assay kit. The BDNF antibody reacts against mature BDNF as well as pro BDNF. Results shown in this study correspond to the 14 KDa band. A control for protein loading was performed by reprobing membranes with an antibody against B actin. No significant changes during the 2 weeks culture period without Inhibitors,Modulators,Libraries drug treatment were ob served for any of the measured proteins. Membranes were incubated with secondary anti mouse or anti rabbit IgG Peroxidase. Immunopositive bands were visualized using the Enhanced Chemiluminescence Plus western blotting system from Amersham. Pictures of the Inhibitors,Modulators,Libraries bands were taken and a subsequent analysis was performed on a Biospectrum AC Imaging System using VisionworksLS software.

Values obtained were normalized and expressed as the ratio obtained from cultures under control conditions. Background Post traumatic stress disorder is an anxiety disorder that develops after exposure to a life threatening traumatic experience, and is characterized by intrusive memories, Inhibitors,Modulators,Libraries a hyper arousal state and avoidance of stimuli associated with the trauma. PTSD is also characterized by changes in the neuroendocrine system including abnormal blood corticosteroid concentration and enhanced negative feedback of the hypothalamic pituitary adrenal axis. Corticosteroids inhibit the HPA axis via 2 types of corticosteroid receptors mineralocorticoid receptors and glucocorticoid receptor. Without corticosterone, unbound MR and GR are believed to be primarily localized in the cytoplasm, but can translocate to the nucleus after binding to the hormone ligand.

Both receptors show different affinity for Inhibitors,Modulators,Libraries corticosteroids. MR has a higher affinity for corticosteroids than the GR. At basal levels of corticosterone, MR is believed selleck inhibitor to be predominant in the maintenance of homeostasis, whereas GR becomes activated when corticosteroid levels increase after stress. Several animal models of PTSD have been developed for mimicking the pathophysiology and behavioral characteristics of PTSD.