53,132 Two main types of invasive recordings are available: subdural grids that are used in the great majority of epilepsy surgery centers worldwide, and depth-electrodes,

including stereoelectroencephalography (SEEG), that are primarily used in French and Italian centers. Both techniques have specific advantages Inhibitors,research,lifescience,medical and drawbacks, and suffer from limited spatial sampling. Subdural grids can buy Cyclopamine provide an accurate delineation of EZ located on the cortical surface of the brain, whereas SEEG appears more appropriate for investigating deeply located EZ, such as in the insula, the mesial aspects of the frontal, temporal, parietal, Inhibitors,research,lifescience,medical and occipital lobe, or the bottom of deep sulci.52,132-136 In any case, the placement of subdural or depth electrodes

is individualized according to all available presurgical data. Complications of these invasive procedures are usually minor and occur in only 1 % to 2% of cases.137 A large number of relevant, information can be provided by intracranial EEG recordings, including interictal slow waves, spikes, and bursts of high frequency oscillations, ictal discharges, and responses to various types of electrical cerebral stimulation. It was recently shown that abnormal high frequency oscillations, including ripples and fast ripples, Inhibitors,research,lifescience,medical either occurring at seizure onset or during interictal bursts, were the most reliable marker of the EZ.138-141 High- and low-frequency electrical stimulation of the Inhibitors,research,lifescience,medical suspected EZ can be used to reproduce the patient’s ictal signs and EEG discharges, and to test eloquent cortex. Conclusions Thanks to the advances of many investigations, an increasing number of patients with drug-resistant epilepsy can benefit, from a conclusive presurgical evaluation that will hopefully lead to a successful surgical treatment. Nevertheless, important progress still needs to be achieved in Inhibitors,research,lifescience,medical order to assess the performance and specific impact, of these Parvulin various

investigations more rigorously. Large multicenter randomized controlled trials should be the method of choice whenever possible. Such trials are likely to promote more homogeneous presurgical strategy among centers and countries. In turn, harmonization of practice should result in increasing the yield of successful epilepsy surgery. Dissemination of current knowledge regarding the eligibility criteria for entering a presurgical evaluation and the success rate of epilepsy surgery represents another major challenge in the field. ‘IMs should allow more patients with refractor}’ seizures to benefit from a timely and effective surgical cure of their devastating disease.