H 89 concentration aureus cultures at different incubation times cfu*ml-1 optical density 600 nm S. aeruginosa Time point average stdev average stdev average stdev average stdev 0 h 4.04E + 5 2.75E + 5 2.17E + 06 5.13E + 05 0.0291 0.0134 0.047 0.008 1 h 30 m 2.38E + 6 1.63E + 6 9.76E + 06 3.33E + 06 0.0349
0.0111 0.051 0.005 2 h 15 m – - 1,83E + 07 6.13E + 06 – - 0.058 0.005 find more 3 h 00 m 7.38E + 6 3.73E + 6 6.17E + 07 2.33E + 07 0.0652 0.0076 0.066 0.005 3 h 45 m – - 1.18E + 08 6.32E + 07 – - 0.077 0.012 4 h 30 m 4.95E + 7 2.91E + 7 1.61E + 08 7.35E + 07 0.1814 0.0190 0.088 0.012 5 h 15 m – - 1.83E + 08 8.12E + 07 – - 0.097 0.012 6 h 00 m 1.30E + 8 4.52E + 7 2.91E + 08 1.19E + 08 0.2531 0.0085 0.101 0.015 24 h 00 m – - 2.31E + 09 1.02E + 09 – - 0.511 0.138 26 h 00 m – - 4.64E + 09 1.35E + 09 – - 0.813 0.133 28 h 00 m – - 5.91E + 09 2.46E + 09 – - 0.892 0.109 A high number of different VOCs were found to be released by both bacterial species in a concentration range varying from part per trillion (pptv) to part per million (ppmv). aureus released 32 VOCs of diverse chemical classes amongst which 28 were analyzed in Selected Ion Monitoring
mode (SIM) and 4 in Total Ion Chromatogram SGLT inhibitor mode (TIC), comprising 9 aldehydes, 4 alcohols, 3 ketones, 2 acids, 2 sulphur containing compounds, 6 esters and 6 hydrocarbons. Table 2 Median concentrations of VOCs released or consumed by Staphylococcus aureus median concentrations [ppbv] Compound CAS m/z for SIM medium 1.5 h 3.0 h 4.5 h 6.0 h propanal 123-38-6 57 3.955 10.62 14.22 8.932 7.04 3-methyl-2-butenal 107-86-8 55, 84 1.526 1.832 3.415 Galactosylceramidase 5.708 5.348 2-ethylacrolein 922-63-4 84 1.656 2.01 6.453 5.537 5.775 (Z)-2-methyl-2-butenal 1115-11-3 84 73.48 81.91 177.4 268.5 247.9 (E)-2-methyl-2-butenal 497-03-0 84 < LOD < LOD 0.259 0.394 0.381 benzaldehyde § 100-52-7 107 20.64 19.08 17.65 12.66 3.815 methacrolein 78-85-3 70 5.922 5.644 9.328 7.617 6.36 acetaldehyde 75-07-0 43 528.5 606.4 374.2 1022.7 1417.4 3-methylbutanal ** 590-86-3 – 317.1 403.3 2764.3 4779.3 4818.5 2-methylpropanal ** 78-84-2 − 598.6 658.5 2044.5 1698.6 1299.5 1-butanol 71-36-3 56 < LOD < LOD < LOD 21.24 59.4 2-methyl-1-propanol 78-83-1 56, 74 0 0 0 21.32 52.62 3-methyl-1-butanol 123-51-3 55, 70 0 0 0 27.65 210.0 ethanol ** 64-17-5 – 0 89.57 237.0 6173.0 11695.1 acetoin (hydroxybutanone) 513-86-0 88 < LOD 3.59 8.004 140.6 279.3 acetol (hydroxyacetone) 116-09-6 74 < LOD < LOD < LOD 113.5 331.0 2,3-butanedione 431-03-8 86 22.65 23.92 27.45 49.84 67.99 acetic acid 64-19-7 45, 60 0 0 0 880.5 2566.6 isovaleric acid 503-74-2 60 0 0 0 31.13 97.