b. brucei and T. congolense. Significantly, the by-products displayed lower trypanocidal activities than pure ISM, since the two isomers and the disubstituted compound had IC50 values approximately 10-fold and 118-fold higher than ISM respectively against T. congolense. For this reason, the presence of these by-products at high quantities (the red and blue isomers may together constitute up to 40% of the final product, ( Schad et al., 2008) in commercial preparations of ISM is concerning given the prevalence

of ISM-resistant T. congolense strains ( Delespaux et al., 2008). Interestingly, the in vitro results indicated that T. b. brucei was 15-fold less sensitive to ISM than T. congolense. Since it is known that a difference in mitochondrial energy metabolism existing among trypanosomatids ( Tielens and van Hellemond, 2009); and that the mitochondrial electrical potential may play a role in ISM uptake ( Wilkes et al., 1997); this Talazoparib nmr Selleck SAR405838 could explain the different level of sensitivity between T. congolense and T. b. brucei. Two different doses of the compounds (0.1 and 1 mg/kg) were used for the in vivo studies to approximate the range of the doses used in the field ( Diarra et al., 1998), either for trypanocidal treatment or prophylaxis, which also differs depending on the sensitivity of the strain ( Gray et al., 1993, Peregrine et al., 1988 and Wilkes

et al., 1997). Since the commercial products may contain as little as 6% of some of the by-products (disubstituted compound), the lower dose (0.1 mg/kg) is more representative of dose rates achieved for the by-products under field conditions when animals are dosed at 0.5–1 mg/kg with the commercial mixtures. In terms of trypanocidal effect, the in vivo first results at the doses tested with the ISM-sensitive strain, confirmed the in vitro tests to the extent that all the compounds tested were active except for

the disubstituted compound at a lower concentration The disubstituted compound had an IC50 118-fold higher than ISM in vitro, therefore, it was not surprisingly that higher dose would be required for a similar level of trypanocidal activity to ISM in vivo. ISM, Veridium®and Samorin® and the disubstituted compound (at 1 mg/kg only) showed similar prophylactic activities against T. congolense challenge in mice in vivo. This prophylactic activity achieved at 1 mg/kg with the disubstituted compound could be explained by the fact that the disubstituted compound acts like a pro-drug and may be cleaved in vivo to produce ISM. Although the disubstituted compound was known to be prophylactic ( Brown et al., 1961), the current study demonstrated that this activity is highly dose-dependent. For this reason, it is crucial to note that a standard dose of commercial ISM products contains less than 0.1 mg/kg of the disubstituted compound ( Schad et al., 2008), which would be insufficient for a trypanocidal effect.