Competing interests The authors declare that they have no competing interests. Authors’ contributions ST and MN designed experiments, interpreted data and drafted the manuscript; TH and HT managed patient samples, Nilotinib Leukemia prepared RNA; ZW and XW performed real-time PCR; YO, JH, YI and GS provided detailed ideas and discussions. All authors have read and approved the final manuscript. Supplementary Material Additional file 1: CRP mRNA expression and CRP protein level. Description: Depiction of the diagnostic accuracy of CRP and mRNA levels. (a) Change in circulating CRP mRNA expression during the clinical course in ICU. Upregulation of CRP mRNA was induced at POD 1 by the surgical intervention. The longitudinal axis is relative CRP mRNA expression compared with ��-actin mRNA in serum. (b) ROC curve analysis.
Bold solid line, bold dotted line, and dotted line refer to CRP level, CRP mRNA and reference, respectively. (c) AUC of the ROC curve analysis of each biomarker. The sensitivities of CRP level and CRP mRNA were 98.6% and 74.1%, respectively. CRP level was superior to CRP mRNA as an inflammatory biomarker. Click here for file(85K, TIFF) Additional files 2: Correlation between GE and clinical parameters. To examine the relationship between clinical parameters and GE, the Pearson correlation analysis test was performed from POD 0 to POD 14. DVD: duration of ventilator dependence. Click here for file(70K, TIFF) Additional file 3: Surgical treatment and an anastomotic leakage. Surgical treatment and an anastomotic leakage are shown.
Click here for file(61K, TIFF) Additional file 4: Correlation between GE and clinical parameters. To examine the relationship between clinical parameters and GE, the Pearson correlation analysis test was performed from POD 0 to POD 14. DVD: duration of ventilator dependence. Click here for file(71K, TIFF) Acknowledgements This study was financially supported by a Grant-in-Aid for Scientific Research from the Ministry of Education, Science. No conflicts of interest.
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