Review of mycorrhiza A change of the common mycological staining method was used to clear and spot products. The ramification of the branches was also considered, the lengths of all the major branches rising from the soil, along with the lengths of all of the side branches, were measured and evaluated. Great roots were tested, while knotweed LY2484595 roots were hand separated from the roots, and both were stained and examined for the presence of mycorrhiza. The test was ended following the second year in September 2007. At the conclusion of the test, the above-ground and below-ground biomass were measured, the fine roots were tested for mycorrhiza and greater roots and rhizomes were thoroughly cleaned using water and air stress. These were then dried and ground for analysis. Melilot was permitted to develop without restriction during the initial season, but plants were repeatedly cut during the 2nd season to keep up a height of 30 cm. Field test The centre of the 1 ha experimental low irrigated area is at an area of fifty 35 N, 13 52 E. This test field is just a former spoil bank that was transformed into an arable field by organic manuring Urogenital pelvic malignancy and ploughing and still shows a top clay content. In April 2006, 15-20 cm long rhizomes of pre developed Dhge. bohemica were grown with a spacing of 100 70 cm and were immediately covered with soil. Ten crops were randomly sampled on each testing time in July and September of 2006, and in July, May and September of 2007 and 2008. Plants were then washed and dried aboveground and the belowground biomass was measured. Whilst the samples from the pot experiment Si samples from each set were analysed for exactly the same stilbenes and emodin. Normal analyses The stilbenes MAPK phosphorylation resveratrol, piceatannol and its glycosides, were analysed together with emodin in samples of roots and knotweed rhizomes. Dry and finely ground samples were extracted with 60% ethanol, and the components were analysed using HPLC. Fig. 13 shows a typical record of the stilbenes and emodin calculated by this method. The soil samples were washed with water on a sieve. The sources were handseparated, cut in to 1 2 cm pieces, washed with one hundred thousand KOH solution and stained with 0. 05% trypan blue in lactoglycerol. Root portions were seen under a microscope at 100 or 200 magnification and were screened for mycorrhizal colonisation. The presence or absence of AM colonisation was established. The amount of mycorrhizal colonisation was assessed using the grid line intersect technique at 50 magnification under a dissecting microscope. The frequency and intensity of mycorrhizal colonisation were also calculated. Data analysis The data were analysed using SPSS 15. 0 statistical software. Normality of the data was tested and non normally distributed data were transformed by position.