For example, the rs2623047 G>A showed an association with age of

disease onset (Table 3); the patients with the AA genotype had a mean age of selleck compound onset of 65.0 ± 9.9 years; and those with the AG genotype had 61.2 ± 10.8 years, while those with the rs2623047 GG showed 56.8 ± 10.7 year age of onset (P = 0.027 for the ANOVA test). The trend test showed a P value of 0.007 for a decreasing age with the G allele in a dose-dependent manner (Table 3). The rs13264163 AG heterozygotes also showed the youngest age of onset among all genotypes of rs13264163A>G (P = 0.016) (Table 3). We also found that the early age of disease onset was associated with the G allele of rs6990375 G>A [rs6990375 GG: 60.0 ± 10.7 years; rs6990375 GA: 61.8 ± 10.6 years; rs6990375 AA: 69.1 ± 9.0 years (P = 0.013)] (Table 3). As we noticed in the LD analysis, rs6990375 G>A had a r 2> 0.8 with

rs3802278 G>A and rs3087714 C>T; therefore, we also observed the significant trends in differences of age of disease onset among genotypes of rs3802278 G>A and rs3087714 C>T (P trend = 0.021 and 0.041, respectively), even though the differences were not significant in ANOVA tests (P = 0.069 and 0.119). Table https://www.selleckchem.com/products/z-ietd-fmk.html 3 SULF1Genotype distribution and age of disease onset Genotypes Number of patients (%) Age at diagnosis (years, mean ±SD) b P-value rs2623047 G>A a     0.027    GG 16 (11.9) 56.8 ± 10.7      GA 80 (59.3) 61.2 ± 10.8      AA 39 (28.9) 65.0 ± 9.9      G allele frequency 112 (41.5)   P trend c = 0.007    A allele frequency 158 (58.5)     rs13264163 A>G     0.016    AA 70 (51.4) 63.7 ± 10.5      AG 53 (39.0) 58.6 ± 10.5      GG 13 (9.6) 64.9 ± 10.6 Tenoxicam      A allele frequency 193 (71.0)   P trend c = 0.266    G allele frequency 79 (29.0)     rs6990375 G>A  

  0.013    GG 58 (42.7) 60.0 ± 10.7      GA 63 (46.3) 61.8 ± 10.6      AA 15 (11.0) 69.1 ± 9.0      G allele frequency 179 (65.8)   P trend c = 0.009    A allele frequency 93 (34.2)     rs3802278 G>A     0.069    GG 59 (43.4) 59.7 ± 11.4      GA 65 (47.8) 62.8 ± 10.0      AA 12 (8.8) 66.7 ± 9.5      G allele frequency 183 (67.3)   P trend c = 0.021    A allele frequency 89 (32.7)     rs3087714 C>T     0.119    CC 63 (46.3) 60.1 ± 11.3      CT 62 (45.6) 62.7 ± 10.1      TT 11 (8.1) 66.6 ± 10.0      C allele frequency 188 (69.1)   P trend c = 0.041    T allele frequency 84 (30.9)     a One sample failed in this genotype b One-way ANOVA (Analysis of variance) for age differences among 3 genotypes for each SNP c P values for the trend test of age at diagnosis among 3 genotypes for each SNP from a general linear model We further evaluated the combined allele effect on age of disease onset.