Furthermore, as discussed previously, the β-Glucosidases, in the

Furthermore, as discussed previously, the β-Glucosidases, in the presence of glucose on a rich medium, as the wine, are able to modulate the response of many compounds, such as, the transference of the glucose molecule to the tyrosol to form salidroside. On the other hand, salidroside may be degraded into tyrosol and glucose ( Ling-Ling, Zhu, Petrovic, & Gonsalves, 2007). More studies will be performed to corroborate this hypothesis, because to our knowledge, the salidroside in wines has not been demonstrated until now. The contents Atezolizumab of gallic acid (Fig. 3b) into CHC and CHA samples showed a tendency to increase during the sur lie. This possibility can be related with the enzymes released during yeast autolysis that could be involved

in the hydrolysis of tannins polymers ( Pozo-Bayón et al., 2009). This result is reinforced by the positive correlation observed between the sur lie and gallic acid (CHC: R = 0.659, p = 0.01; CHA: R = 0.603, Smad inhibitor p = 0.01).The content was similar to the one observed in Cavas and white wines ( Bosch-Fusté et al., 2009 and Esteruelas et al., 2011), and higher than

in Champagnes ( Vauzour et al., 2010). On the contrary, the gallic acid curve at ageing on lees in CTA samples shows a tendency to decrease, although the level has remained in an average range in comparison to the other analysed groups. Since gallic acid is a monomer of the tannins, in the Charmat process the OPC hydrolysis can be hindered due to the fact that surface contact between the wine

and the lees is smaller. Positive correlation between OPC and gallic acid was observed only in this type of SW (CTA: R = 0.484, p = 0.01).The differences observed on the gallic acid curves are linked with the response of the antioxidant capacity assay ( Table 2). Higher levels of caffeic acid (Fig. 3c) were obtained in CHA and CTA samples, indicating a strong influence of the varieties in the concentration of this phenolic compound. Our data is higher than what was observed in Cavas ( Bosch-Fusté et al., 2009), but similar to other white wines ( Esteruelas et al., 2011). The presence of caffeic acid was observed in all samples and the curve during the sur lie was similar and constant for the three analysed groups. This aspect is very important, because the browning increase is due to the formation of brown macromolecules coming from the polymerisation (-)-p-Bromotetramisole Oxalate of phenols; the decrease in the main hydroxycinnamic acids present in SW is also related with these reactions and can affect the overall quality ( Bosch-Fusté et al., 2009). Moreover, the caffeic acid associate with proteins creates an initially soluble molecule, but with the growth, the complex becomes insoluble, generating turbidity into wines ( Esteruelas et al., 2011). Additionally, the degree of insolubility is affected by the nature of the sugars present in the medium and in these samples, negative correlation between caffeic acid and glucose was observed (CHC: R = −0.446, p = 0.05; CHA: R = −0.477, p = 0.

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