Key Word(s): 1. adaptive biofeedback; 2. constipation; 3. pathogenesis; Presenting Author: HUA GAO

Additional Authors: XINCAI XU, FENG WANG, WENBING ZHANG, YUNHAI WANG Corresponding Author: YUNHAI WANG Affiliations: Gastrointestinal surgery department, 1st teaching hospital of Xinjiang medical university Objective: Anterior gradient 2 (AGR2) is said to be a prognosis factor in the colorectal cancer patients. Its expression in the serum shows an important role in the occurrence, development and metastasis of colon cancer. However, the mechanism is still unclear. Methods: We designed and constructed the expression plasmids with small interfering RNA (siRNA) aiming at AGR2, then transfected into colon BGB324 cell line cancer cell line this website SW620 by eukaryocyte transfection technique. In the research, we compared the cell characters of AGR-siRNA group with the control groups, the cell line and the AGR-siRNA vacant group. The protein expressions of AGR2 were detected by Western blotting. Cellular proliferation and invasion character were analyzed by tetrazolium bromide colorimetry (MTT)

and cell migration assay respectively. And the expression of the gene and protein of vascular endothelial growth factor (VEGF) and S100 calcium binding protein A4 (S100A4), which were identified as prognosis factors, also tested by real time PCR and Western blotting. Results: Enzymatic digestion and Branched chain aminotransferase DNA sequencing confirmed that the AGR2 specific siRNA expression plasmids were constructed successfully. After plasmids were transfected into cells, the protein expressions of AGR2 was significantly down-regulated as compared with control groups (P < 0.01). The cellular proliferation inhibitory rates in AGR2 siRNA group was higher than that

in control groups (P < 0.05). The cell invasion rates in AGR2 siRNA group was lower than that in control groups (P < 0.05). The gene and protein expressions of VEGF and S100A4 were significantly down-regulated respectively as compared with control groups (P < 0.01). Conclusion: Our results indicate that the expression of AGR2 is important to the growth of CRC, and that it may promote progression by regulating VEGF and S100A4 expression. Key Word(s): 1. Anterior gradient 2; 2. si-RNA; 3. VEGF; 4. S100A4; Presenting Author: JING YANG Additional Authors: YUNSHENG YANG, SHUNTAN CAI, LIHONG CUI, LIHUA PENG Corresponding Author: YUNSHENG YANG Affiliations: Department of Gastroenterology and Hepatology, Chinese PLA General Hospital Objective: Epidemiological characteristics of IBS in military race maybe quite different from the civilians, but the exact number is unknown. So we conduct a study to investigate the prevalence and related factors of IBS followed subjects of the military race.