maris Coryn-1T [8], C marinum 7015T [9] and C humireducens MFC-

maris Coryn-1T [8], C. marinum 7015T [9] and C. humireducens MFC-5T [10] as well as C. diphtheriae NCTC 11397T [11] indicates that http://www.selleckchem.com/products/Vandetanib.html C. halotolerans YIM 70093T, together with C. maris, C. marinum, and C. humireducens, form a distinct subclade within the genus Corynebacterium. Interestingly, C. xerosis and C. freneyi do not group closely with this subclade when C. diphtheriae is added to the comparison. Figure 1 shows the phylogenetic neighborhood of C. halotolerans in a 16S rRNA based tree. The sequences of the four identical 16S rRNA gene copies in the genome differ by eight nucleotides from the previously published 16S rRNA sequence (“type”:”entrez-nucleotide”,”attrs”:”text”:”AY226509″,”term_id”:”29372859″,”term_text”:”AY226509″AY226509), which contains two ambiguous bases.

Figure 1 Phylogenetic tree highlighting the position of C. halotolerans relative to type strains of other species within the genus Corynebacterium as selected by Chen et al. [1]. In addition, the recently described C. maris, C. marinum, and C. humireducens were … C. halotolerans YIM 70093T is Gram-positive and cells are rod-shaped, 0.5-1 ��m long and 0.25-0.5 ��m wide (Table 1 and Figure 2). It is described to be non-motile [1], which coincides with a complete lack of genes associated with ��cell motility�� (functional category N). Optimal growth of YIM 70093T was shown to occur at 28��C, pH 7.2 and 100 g/l KCl, albeit the strain tolerates a wide range of salinity, between 0-250 g/l, NaCl, and MgCl2 [1].

Carbon sources utilized by strain YIM 70093T include glucose, galactose, sucrose, arabinose, mannose, mannitol, maltose, xylose, ribose, salicin, dextrin, and starch [1], although the latter is doubtful as C. halotolerans cannot hydrolize starch [1]. Table 1 Classification and general features of C. halotolerans YIM 70093T according to the MIGS recommendations [14]. Figure 2 Scanning electron micrograph of C. halotolerans YIM 70093T. Chemotaxonomy The peptidoglycan of strain YIM 70093T contains meso-diaminopimelic acid, galactose, and arabinose [1], therefore it belongs to cell wall type IV, sugar type A. The menaquinones detected in the cell membrane of YIM 70093T are MK-8(H2) (35.5%) and MK-9(H2) (64.5%) [1]. Cellular fatty acids are predominantly saturated straight chain acids, C16:0 (42.1%), C14:0 (7.3%); and C18:0 (4.5%), and unsaturated acids, cis-9-C18:1 (28.

9%) and cis-9-C16:1 (9.8%), in addition to 10-methyl C18:0 (7.4%) [1]. Like many, but not Entinostat all corynebacteria, C. halotolerans also contains mycolic acids, predominantly of the short chain type (C32-C36): C32:0 (36.0%), C34:0 (20.8%), C34:1 (25.1%), C36:0 (3.6%), C36:1 (8.4%), and C36:2 (5.1%) [1]. The reported major polar lipids consist of diphosphatidylglycerol (DPG), phosphatidylglycerol (PG), phosphatidylinositol (PI), glycolipid and phosphatidylinositol mannosides (PIM) [1]. Genome sequencing and annotation Genome project history C.

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