Microbiology 1998, 144:975–983 PubMedCrossRef 24 Schuster CB, Do

Microbiology 1998, 144:975–983.PubMedCrossRef 24. Schuster CB, Dobrinski B, Hakenbeck R: Unusual septum formation in Streptococcus pneumoniae mutants with an alteration in the D, D-carboxypeptidase penicillin-binding proteins 3. J Bacteriol 1990, 172:6499–6505.PubMed 25. Kozarich JW, Strominger JL: A membrane enzyme from Staphylococcus aureus which catalyzes transpeptidase, carboxypeptidase, and penicillinase activities. J Biol Chem 1978, 253:1272–1278.PubMed 26. Kimura Y, Takashima Y, Tokumasu Y, Sato M: Molecular cloning, sequence analysis, and

characterization of a penicillin-resistant DD-carboxypeptidase of Myxococcus xanthus . J Bacteriol 1999, 181:4696–4699.PubMed 27. Denome SA, Elf PK, Henderson TA, Nelson DE, Kevin D, Young KD: Escherichia coli mutants lacking all possible combinations of eight penicillin binding proteins: viability, characteristics, and implications this website for peptidoglycan

synthesis. J Bacteriol 1999, 181:3981–3999.PubMed 28. Stefanova ME, Tomberg J, Olesky M, Höltje JV, Gutheil WG, Nicholas RA: Neisseria gonorrhoeae penicillin-binding protein 3 exhibits exceptionally high carboxypeptidase and beta-lactam binding activities. Biochemistry 2003, 42:14614–14625.PubMedCrossRef 29. Popham DL, Gilmore ME, Setlow P: Roles of low-molecular-weight penicillin-binding proteins Pritelivir ic50 in Bacillus subtilis spore peptidoglycan synthesis and spore properties. J Bacteriol 1999, 181:126–132.PubMed 30. Ghosh AS, Chowdhury C, Nelson DE: Physiological functions of D-alanine carboxypeptidases in Escherichia coli . Trends Microbiol 2008, 16:309–317.PubMedCrossRef 31. Camilli A, Tilney LG, Portnoy DA: Dual roles of plcA in Listeria monocytogenes pathogenesis. Mol Microbiol 1993, 8:143–157.PubMedCrossRef 32. Park SF, Stewart GSAB: High-efficiency transformation of Listeria monocytogenes C59 ic50 by electroporation of penicillin-treated cells. Gene 1990, 94:129–132.PubMedCrossRef 33. Frere JM, Leyh-Bouille M, Ghuysen JM, Nieto M, Perkins

HR: Exocellular DD-carboxypeptidases- transpeptidases from Streptomyces . Methods Enzymol 1976, 45:610–636.PubMedCrossRef 34. Glauner B: Separation and performance liquid chromatography. Anal Biochem 1988, 172:451–464.PubMedCrossRef 35. Hayashi H, Araki Y, Ito E: Occurrence of glucosamine residues with free amino groups on cell wall peptidoglycan from Bacillus as a factor responsible for resistance to lysozyme. J Bacteriol 1973, 113:592–598.PubMed Authors’ contributions DK carried out the molecular cloning, recombinant protein expression and protein purification as well as the physiological characterization of the obtained mutants, and helped to draft the manuscript. ZM conceived part of the study, participated in its design and coordinated the preparation of the manuscript. GOG conceived part of the study and collaborated in preparation of the manuscript.

Leave a Reply

Your email address will not be published. Required fields are marked *

*

You may use these HTML tags and attributes: <a href="" title=""> <abbr title=""> <acronym title=""> <b> <blockquote cite=""> <cite> <code> <del datetime=""> <em> <i> <q cite=""> <strike> <strong>