Planistromella A.W. Ramaley, Planistroma A.W. Ramaley, Mycosphaerellopsis Höhn.,

and Comminutispora A.W. Ramaley with their asexual states appear to belong in Botryosphaeriaceae J. Monkai et al. pers. comm.). Otthia (Cooke 1871, 1890; Massee 1887; Stevens 1936; Bisby and Mason 1940) which was introduced from Ulmus sp., with six species, but without a generic type being named (Fuckel 1870), might be considered for inclusion in Botryosphaeriaceae. Booth (1958) selected a lectotype in O. spiraeae and considered Diplodia sarmentorum (Fr.) Fr. to be the asexual morph. Phillips et al. (2005) redescribed and illustrated Otthia spiraeae and placed Diplodia Emricasan mouse sarmentorum in a new species named Botryosphaeria sarmentorum A.J.L. Phillips, Alves & Luque.

They considered the holotype of Otthia spiraeae and the specimen illustrated by Booth (1958) to be from different genera, with O. spiraeae having cylindrical asci with a thin endotunica, while Booth’s specimen (Fig. 1 in Booth 1958) had clavate asci with a thick endotunica more typical of Botryosphaeriaceae. Schoch et al. (2009a) sequenced two strains named Otthia spiraeae from CBS (isolated from Ulmus glabra by K. & L. Holm in 1987, Sweden, Herbarium, UPS) and these clustered in Botryosphaeriaceae (see Fig. 1). However, it is not clear whether the strains used in Schoch et al. (2009a) were correctly identified and therefore the placement of Otthia (synonym = Otthiella LY2090314 (Sacc.) Sacc. & D. Sacc., Syll. Fung. (Abellini) 17: 662 1905) in Botryosphaeriaceae cannot be confirmed until fresh collections identical to the holotype are made and sequenced. It is evident however, that the Dothiorella Clade (Fig. 1, Clade A6) in our study, which includes the sequences from putative Otthia species, is a distinct genus. The asexual morphs Dolichyl-phosphate-mannose-protein mannosyltransferase of Botryosphaeriaceae include species with brown, unicellular or bi-celled conidia (Aplosporella, Diplodia, Dothiorella, Macrophomina,

Neoscytalidium and Lasiodiplodia) and species with hyaline conidia (Fusicoccum, Neofusicoccum and Pseudofusicoccum). In Table 2 we list the sexual morph against the asexual morph and provide an argument for which name should be used now that only a single name is available for each genus and taxon. Each plate was inoculated with more than three (generally five) single ascospores, derived cultures. We ensured this primarily to obtain secondary or dikaryotic mycelium, which enhanced the formation of sexual or asexual morphs. It is evident that several groups of botryosphaeriaceous taxa are species complexes and these need to be resolved using multi-gene sequence analysis which should include protein genes. For example, the genus Lasiodiplodia is likely to comprise several species complexes (Burgess et al. 2006; Alves et al. 2008; Abdollahzadeh et al. 2010). Other genera which may also comprise species complexes are Aplosporella, Botryosphaeria, Dothiorella, Neofusicoccum and Spencermartinsia (Phillips et al. 2005; Crous et al.