(PPT 187 KB) Additional file 2: PCR confirmation of epitope insertion in the recombinant phage. The inserted epitope fragment in recombinant M13KE was confirmed by colony PCR. M is the DNA ladder. 1 is the fragment amplified
from wild type phage M13KE, 2-5 are the epitope fragments 59-78, 87-98, 173-191 and 297-320 of OmpL1. 6-9 are the epitope fragments 30-48, 181-195, 233-256 and 263-282 of LipL41. (PPT 195 KB) References 1. McBride AJ, Athanazio DA, Reis MG, Ko AI: Leptospirosis. Curr Opin Infect #Bcl-2 inhibitor randurls[1|1|,|CHEM1|]# Dis 2005, 18 (5) : 376–386.PubMedCrossRef 2. Palaniappan RU, Ramanujam S, Chang YF: Leptospirosis: pathogenesis, immunity, and diagnosis. Curr Opin Infect Dis 2007, 20 (3) : 284–292.PubMedCrossRef 3. Lindenstrøm T, Agger EM, Korsholm KS, Darrah PA, Aagaard C, Seder RA, Rosenkrands I, Andersen P: Tuberculosis subunit vaccination provides long-term protective immunity characterized by multifunctional
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