some granulated metrial gland cells showed immunoreactivity. the mesometrial decidua has attained its utmost development, the definitive placenta has formed in the region and invasion of the central artery has already happened. The three apoptotic markers were discovered within the decidual cells situated in the areas close to the ectoplacental cone and surrounding the bloodstream occupied by the endotrophoblasts, which were contact us negative. Although Bax and Bcl 2 expression was more intense inside the horizontal mesometrial decidua, however less than day 10 of gestation. The intensity of the signal was decreasing in the way of the circular muscle coat. The smooth muscle of the arteries and the dispersed circular muscle layer continued to be immunoreactive for BclxL and Bax and extremely low levels were also observed for Bcl 2. All the apoptogenic elements were also present in low levels within the longitudinal muscle layer within the mesometrial side. In-the metrial gland some round and granulated metrial gland cells localized between the distributed muscle layer were immunopositive for all your factors. O-n day 14 the pat-tern of expression for the different facets was much like day Organism 12 in-the decidua, though Bcl 2 was less powerful. In the metrial gland the term for the anti apoptotic markers was related, on the contrary to day 1-2 positive granulated metrial gland cells were spread throughout the gland. Some distributed muscle cells inside the metrial gland were positive for Bax. The longitudinal muscle layer in-the mesometrial side was highly positive for the factors. The brand new epithelium in development remained good for both anti apoptotic markers. Days 1-6 to 19 After day 1-4 the mesometrial decidua has regressed forming the decidua basalis. Within this time, the pattern of expression resembles the last days. Within the metrial gland Bcl xL was extinguished and though the expression of the pro apoptotic sign in relation to Bcl 2 was more powerful, a decrease (-)-MK 801 in-the expression of the other facets was found. While the expression in the longitudinal layer was much like day 1-4, although a decrease in expression was observed for Bcl xL, the dispersed circular muscle layer remained good for Bax. By day 1-9 the expression of the anti apoptotic element remained in a couple of isolated cells in the decidua basalis and in very low levels in the metrial gland. Bax immunoreactivity was detected in the same regions though with stronger power. Some immunopositive cells were possessed by the thin circular muscle coat for Bax. Moreover, a decreased expression for Bcl 2 and Bax was present in the longitudinal layer. Caspase actions in-the homogenates, from days 10, 1-2, 14 and 1-6 of pregnancy, were examined spectrophotometrically with synthetic peptides as substrates.