The Andreev reflection current also exhibits a spin polarization
under the influence of both the spin-orbit interaction and the magnetic field through the ring. The spin polarization of the current can be tuned by varying the spin-orbit interaction strength and the magnetic flux. This provides an efficient mechanism to control https://www.selleckchem.com/products/cobimetinib-gdc-0973-rg7420.html the spin accumulation and the Andreev reflection in the quantum dot. (C) 2011 American Institute of Physics. [doi: 10.1063/1.3611398]“
“This work explores the possibility of using a polyethylene glycol (PEG)/sodium citrate aqueous two-phase system (ATPS) as a first step in a process for the purification of lectin from Canavalia grandiflora seed. Purification of lectins is a limiting step for its uses in the area of biotechnology. Extraction
by an aqueous two-phase see more system is a powerful technique for separation, concentration, and purification of biomolecules and pharmaceutical products. Four factors (PEG’s molar mass, PEG’s concentration, pH. citrate concentration) affecting the lectin ConGF partitioning were studied. A two-level factorial design (2(4)) was carried out. Lectin ConGF preferentially partitioned to the top (polyethylene glycol) phase. The statistical analysis showed that the citrate concentration chloride significantly affects the K(L) (partitioning coefficient for protein) value for lectin partitioning. An ATPS composed of 20% (w/w) PEG 400 and 20% (w/w) citrate, at pH 6, allowed the recovery of Selleckchem CA3 lectin ConGF with an 8.67 partition coefficient and 104% yield. Consequently, the system proved to be efficient and can be used as a first step in lectin purification from crude extract of ConGF seeds. (C) 2010 Elsevier B.V. All rights reserved.”
“Background: Delayed hypersensitivity is responsible for severe cutaneous adverse drug reactions (cADRs), especially in Stevens-Johnson syndrome (SJS), toxic epidermal necrolysis, and drug-induced hypersensitivity syndrome (DIHS) (also known as drug rash with eosinophilia and systemic symptoms [DRESS] syndrome). The drug-induced lymphocyte stimulation test (DLST), or lymphocyte transformation test (LTT), is
used to identify the culprit drug in severe cADR cases.
Objective: The aim of this study was to examine the immune reactions in cADR patients through the identification of the drug-specific proliferating cells by flow cytometric DLST (FCM-DLST).
Methods: The peripheral blood mononuclear cells of 16 anticonvulsant-induced cADR patients were investigated by conventional DLST and a FCM-DLST protocol in which CFSE dilution and BrdU incorporation were combined. FCM-DLST allowed for the identification of the drug-specific proliferating cells in six cases. Three of these cases were DIHS cases, whereas there was one case of SJS, one case of maculopapular rash (MP), and one case of erythema multiforme (EM) among the six cases.