Blueberry extracts have demonstrated substantial antimicrobial activity, effectively targeting numerous potential pathogens. The interaction of these extracts with beneficial bacteria (probiotics), especially in relation to food applications, deserves consideration, as their importance extends beyond being a vital component of the normal gut microflora to include their importance as ingredients in standard and functional foods. Hence, this investigation first endeavored to demonstrate the inhibitory impact of a blueberry extract on four potential foodborne pathogens. Further investigation, after identifying the effective concentrations, determined their effects on the growth and metabolic processes (organic acid production and sugar consumption) of five possible probiotic microorganisms. Despite the extract's ability to inhibit the growth of L. monocytogenes, B. cereus, E. coli, and S. enteritidis at 1000 grams per milliliter, it had no impact on the growth of the potential probiotic strains tested. The results, for the first time, clearly demonstrate a significant effect of the extract on the metabolic activity of all probiotic strains, yielding higher amounts of organic acids (acetic, citric, and lactic) and an earlier production of propionic acid.
Films possessing high stability, composed of carrageenan and agar (A-CBAL), were fabricated by incorporating anthocyanin-loaded liposomes to enable non-destructive shrimp freshness monitoring. The liposomes, loaded with anthocyanin, saw their encapsulation efficiency surge from 3606% to 4699% as the lecithin proportion escalated. The water vapor transmission (WVP) of A-CBAL films, at 232 x 10⁻⁷ g m⁻¹ h⁻¹ Pa⁻¹, was found to be lower than that of the A-CBA film containing free anthocyanins. At pH 7 and pH 9, after 50 minutes, the A-CBA film's exudation rate was 100%, while the A-CBAL films' exudation rate decelerated to a rate less than 45%. The phenomenon of ammonia sensitivity was slightly decreased due to the encapsulation of anthocyanins. Finally, the liposome-embedded bi-layer films successfully documented shrimp freshness, marked by visible color shifts, easily observed by the naked eye. Films loaded with anthocyanin-encapsulated liposomes exhibit a potential for use in environments with elevated humidity levels, according to these results.
The present research explores the encapsulation of Cymbopogon khasiana and Cymbopogon pendulus essential oil (CKP-25-EO) within a chitosan nanoemulsion, assessing its ability to suppress fungal infestation and aflatoxin B1 (AFB1) accumulation in Syzygium cumini seeds, particularly regarding its cellular and molecular mode of action. The controlled release of CKP-25-EO, encapsulated in chitosan, was validated by the comprehensive DLS, AFM, SEM, FTIR, and XRD analyses. Selleckchem Esomeprazole The CKP-25-Ne exhibited a greater degree of antifungal (008 L/mL), antiaflatoxigenic (007 L/mL), and antioxidant activity (IC50 DPPH = 694 L/mL, IC50 ABTS = 540 L/mL) as compared to the free EO. Cellular ergosterol impediments, methylglyoxal biosynthesis disruptions, and in silico CKP-25-Ne molecular modeling confirmed the cellular and molecular mechanisms underlying the antifungal and antiaflatoxigenic properties. In the in situ context, CKP-25-Ne demonstrated a positive effect on inhibiting lipid peroxidation and AFB1 secretion in stored S. cumini seeds, leaving the sensory profile unchanged. Subsequently, the favorable safety record among higher mammals provides compelling support for employing CKP-25-Ne as a safe and environmentally conscious nano-preservative, protecting against fungal contamination and dangerous AFB1 presence within the food, agricultural, and pharmaceutical industries.
This research investigated the physicochemical quality of honey imported by the United Arab Emirates (UAE) from Dubai ports during the period of 2017 to 2021. 1330 samples underwent a comprehensive examination of sugar constituents, moisture, hydroxymethylfurfural (HMF) concentration, free acidity, and diastase number. Among the honey samples assessed, an impressive 1054 adhered to the Emirates honey standards. However, a significant 276 samples (208 percent) deviated; this non-compliance stemmed from violating one or more quality parameters, likely hinting at adulteration, poor storage, or improper heat application. In non-compliant samples, sucrose levels averaged between 51% and 334%, while glucose and fructose combined ranged from 196% to 881%. Moisture content exhibited a fluctuation between 172% and 246%, HMF levels spanned 832 to 6630 mg/kg, and acidity varied from 52 to 85 meq/kg. Honey samples that did not meet compliance standards were categorized by their country of origin. Selleckchem Esomeprazole Based on the collected data, India demonstrated the highest percentage of non-compliant samples, reaching 325%, in comparison to Germany, which recorded the lowest non-compliance percentage at 45%. Concerning honey samples exchanged across international borders, this study stressed the significance of incorporating physicochemical analysis into the inspection process. A systematic examination of honey at Dubai's ports should contribute to the reduction in imported adulterated products.
To mitigate the risk of heavy metal pollution in infant milk powder, the implementation of robust detection methodologies is paramount. In an electrochemical assessment of infant milk powder, Pb(II) and Cd(II) were detected using a screen-printed electrode (SPE) modified with nanoporous carbon (NPC). NPC's utilization as a functional nanolayer improved the electrochemical detection of Pb(II) and Cd(II), arising from its effective mass transport and high adsorption capacity. Lead (II) and cadmium (II) exhibited linear responses across the concentration ranges of 1 to 60 grams per liter and 5 to 70 grams per liter, respectively. The detectable minimum for lead(II) was 0.01 grams per liter, while cadmium(II) required a concentration of 0.167 grams per liter. The prepared sensor's properties relating to reproducibility, stability, and interference resistance were rigorously tested. The SPE/NPC, a developed method, shows exceptional performance in detecting Pb(II) and Cd(II) heavy metal ions in the extracted infant milk powder sample.
Daucus carota L., widely used as a food source, is noted for its considerable content of bioactive compounds. The process of carrot processing generates residues that are typically discarded or underutilized, offering a chance to develop new ingredients and products, ultimately supporting healthier and more sustainable dietary trends. In the current research, the functional properties of carrot waste powders were evaluated in relation to the effects of various milling and drying procedures, and in vitro digestion. Through a process combining disruption (grinding or chopping), drying (freeze-drying or air-drying at 60 or 70 degrees Celsius), and final milling, carrot waste was transformed into powdered form. Selleckchem Esomeprazole The physicochemical properties of powders, encompassing water activity, moisture content, total soluble solids, and particle size, were examined, alongside the nutraceutical attributes including total phenol content, total flavonoid content, antioxidant activity (by DPPH and ABTS assays), and carotenoid content (?-carotene, ?-carotene, lutein, lycopene). In vitro gastrointestinal digestion's influence on carotenoid and antioxidant levels was also assessed; separate analyses of carotenoids were conducted in different environments: direct, water-based, oil-based, and oil-in-water emulsions. Water activity reduction through processing enabled the creation of powders characterized by high levels of antioxidant compounds and carotenoids. The interplay of disruption and drying methods significantly affected powder characteristics; freeze-drying produced finer powders with elevated carotenoid content, yet lower antioxidant values, in contrast to air-drying, especially of chopped samples, which showcased improved antioxidant activity and higher phenol levels. Digestion, as simulated in vitro, demonstrated the release of bioactive compounds previously held within the powder's structure. Though carotenoid solubilization within oil was unimpressive, the co-ingestion of fat facilitated a substantial rise in carotenoid recovery. Based on the research results, carrot waste powders, containing bioactive compounds, could be identified as promising functional ingredients to elevate the nutritional content of food products, thereby supporting sustainable food systems and healthy eating practices.
An important environmental and industrial challenge involves the recycling of kimchi production waste brine. The waste brine's food-borne pathogens were decreased using an underwater plasma treatment technique. For the treatment of 100 liters of waste brine, capillary electrodes operating with alternating current (AC) bi-polar pulsed power were used. In order to assess inactivation effectiveness, four types of agar were utilized: Tryptic Soy Agar (TSA), Marine Agar (MA), de Man Rogosa Sharpe Agar (MRS), and Yeast Extract-Peptone-Dextrose (YPD). Consistent with a linear trend, the microbial population decreased with treatment time, irrespective of the culturing medium variations. A log-linear model (R-squared of 0.96 to 0.99) described the pattern of inactivation. Salinity, pH, acidity, reducing sugar levels, and microbial counts of plasma-treated waste brine (PTWB) from salted Kimchi cabbage were examined to determine its reusability, contrasting it with the results from newly prepared brine (NMB) and untreated waste brine (WB). The results from PTWB's salted Kimchi cabbage samples presented no substantial distinction in quality from NMB's, confirming the practicality of applying underwater plasma treatment to repurpose wastewater brine in the kimchi salting procedure.
From the earliest days of food preparation, fermentation has been a key strategy for ensuring food safety and increasing its shelf-life. The fermentation process is influenced by starter cultures, predominantly lactic acid bacteria (LAB), which also act as bioprotective agents, controlling native microbiota and the emergence of pathogens. This study explored the potential of LAB strains isolated from spontaneously fermented sausages, originating from varied Italian regions, to act as both starter cultures and bioprotective agents in fermented salami.