Among the positive OMMT

samples, 2 of the 8 specimens showed low positivity (1+) for Trop-2 protein, while the remaining IHC specimens showed moderate (2+ in 3 samples) or strong (3+ in 3 samples) Trop-2 positivity. Without exception, Trop-2 positivity was detected only in the epithelial component of the carcinosarcoma specimens. Figure 1 Representative Trop2 immunostain in ovarian and uterine MMT and control normal tissues. Upper left panel: Strong, diffuse membranous Trop2 expression (3+) in the carcinomatous component of ovarian MMT. Upper right panel: Minimal to absent Trop2 expression in normal ovarian surface epithelium and stroma. Lower left panel: Strong, focal membranous Trop2 expression (2+) in the carcinomatous component Gemcitabine molecular weight of uterine MMT. The adjacent sarcomatous component is negative for Trop2. Lower right panel: Weak, focal Trop2 expression in normal endometrial glands. (All images 200× original magnification) Table 2 IHC Results for Trop-2 Protein Expression in UMMT and OMMT Patients UMMT OMMT PT 1 3+ 0 PT 2 0 3+ PT 3 2+ 2+ PT 4 0 0 PT 5 0 0 PT 6 0 0 PT 7 0 1+ PT 8 0 0 PT 9 2+ 0 PT 10 0 3+ PT 11 3+ 1+ PT 12 0 3+ PT 13 0 2+ PT 14 0 2+ PT 15 1+   PT 16 1+   PT 17 0   PT 18 3+   PT 19 0   PT 20 0   PT 21 0   PT 22 0   PT 23 2+   PT 24 0   PT 25 2+   PT 26 0   Trop-2 Transcript Levels in Carcinosarcomas The ovarian and uterine carcinosarcoma cell lines were tested

for Trop-2 expression selleck chemicals llc by qRT-PCR. Table 1 shows the histopathologic characteristics of the patients from whom the cell lines were KU55933 in vivo established. Trop-2 expression was significantly higher in two cell lines (UMMT-ARK-1 and OMMT-ARK-2)

compared with normal control tissues (Table 3, P = 0.02). Among the carcinosarcomas tested, UMMT-ARK-1 demonstrated a low mRNA relative expression Vildagliptin for Trop-2 (65.7) while OMMT-ARK-2 demonstrated a high mRNA relative expression for Trop-2 (13,032). Negligible Trop-2 expression by qRT-PCR was detected in the other cell lines, with UMMT-ARK-2 and OMMT-ARK-1 having 0.012 and 0.453 mRNA relative expression respectively (Table 3, P = 0.93). Table 3 Trop-2 mRNA and Protein Expression in Carcinosarcoma Cell Lines Cell Line RT-PCR1 Flow cytometry Cells (%) MFI 2     Control NEC3 1 – - Control NOVA4 1 – - UMMT ARK-1 65.7 100 20 UMMT ARK-2 0.5 0 0 OMMT ARK-1 0.1 0 0 OMMT ARK-2 13032 100 151 1RT-PCR – Real-time Polymerase Chain Reaction 2 MFI – Mean Fluorescence Intensity 3 NEC – Normal Endometrial Cells 4 NOVA – Normal Ovarian Cells Trop-2 Surface Expression by Flow Cytometry in Primary Carcinosarcoma Cell Lines To determine whether increased expression of Trop-2 mRNA corresponded with increased Trop-2 cell surface protein expression, we performed flow cytometry on all primary cell lines. Trop-2 surface expression by flow cytometry was found to significantly correlate with Trop-2 mRNA relative expression in all cell lines (Table 3).