In order to validate the accuracy of the reason for discontinuation determined by the clinician, we repeated the analysis with the immunovirological and clinical endpoint,

defining discontinuation as a consequence of failure on the basis of the following: discontinuation Ibrutinib purchase of ≥1 drug in the original regimen concomitant with (i) a single viral load >500 HIV-1 RNA copies/mL, or (ii) an increase in CD4 cell count <10% from the patient's pre-therapy value, or (iii) the occurrence of an AIDS-defining illness. A total of 3291 patients were included in the study: 28.2% were female and 39.9% were HCV antibody-positive; their median age was 36 years [interquartile range (IQR) 32–41 years]. Median

CD4 cell count at HAART initiation was 263 cells/μL (IQR 114–402 cells/μL), and median HIV RNA was 4.8 log10 copies/mL (IQR 4.2–5.3 log10 copies/mL). One hundred and thirty-eight patients (4.2%) initiated therapy with three NRTIs (of whom 117 initiated regimens including abacavir and 21 initiated regimens including tenofovir as the third drug), Nutlin-3a cell line 894 (27.2%) with an NNRTI-based regimen, 366 (11.1%) with a boosted PI, 1786 (54.3%) with a single PI, five (0.1%) with a combination of three other drugs (one NRTI+two PIs) and 102 (3.1%) with CYTH4 four or more drugs. Most patients

(52.6%) started HAART in the early period (1997–1999), 925 (28.1%) in the intermediate period (2000–2002) and 635 (19.3%) in the recent period (2003–2007) (Table 1). The median time of follow-up of patients was 12 (IQR 3–12) months; 288 patients (8.7% of the population) dropped out during the first year of follow-up; 14 of these died. During the first 12 months, 1189 (36.1%) patients discontinued ≥1 drug in their initial HAART. The main causes of discontinuation were intolerance/toxicity (696 of 1189 patients; 58.5%) and poor adherence (285 of 1189 patients; 24%); 126 patients (10.6%) discontinued because of immunovirological or clinical failure and 62 (5.2%) because of simplification strategies. Twenty patients (1.7%) interrupted temporarily or permanently all the ongoing drugs by clinician choice or patient wish. The Kaplan–Meier estimates of drug discontinuation for any reason in the first year were 39.5% (95% CI 37.1–41.9%) in those who initiated in 1997–1999, 35.6% (95% CI 32.3–38.9%) in those who initiated in 2000–2002, and 41.2% (95% CI 37.1–45.3%) in those who initiated in 2003–2007 (log-rank test P=0.06) (Fig. 1).